Abstract
A simple method for the determination of residual oxytetracycline (OTC) in milk by high-performance liquid chromatography (HPLC) was developed. The sample preparation could be made without complex extraction and clean-up procedures. A LiChrospher 100 RP-8 end-capped column and a mobile phase of acetonitrile-acetic acid-water (28:4:68, v/v/v) with a photo-diode array detector was used. The average recoveries from spiked OTC (0.1, 0.5 and 1.0 μg/ml) were in excess of 89.8% with coefficients of variation between 0.6 and 4.1%. The limit of detection was 0.05 μg/ml. The total time required for the analysis of one sample was below 10 min.
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