Abstract
The wide abuse of antibiotics has accelerated bacterial multiresistance, which means there is a need to develop tools for rapid detection and characterization of bacterial response to antibiotics in the management of infections. In the study, an electrochemical biosensor based on nanoporous alumina membrane and graphene quantum dots (GQDs) was developed for bacterial response to antibiotics detection. Anti-Salmonella antibody was conjugated with amino-modified GQDs by glutaraldehyde and immobilized on silanized nanoporous alumina membranes for Salmonella bacteria capture. The impedance signals across nanoporous membranes could monitor the capture of bacteria on nanoporous membranes as well as bacterial response to antibiotics. This nanoporous membrane and GQD-based electrochemical biosensor achieved rapid detection of bacterial response to antibiotics within 30 min, and the detection limit could reach the pM level. It was capable of investigating the response of bacteria exposed to antibiotics much more rapidly and conveniently than traditional tools. The capability of studying the dynamic effects of antibiotics on bacteria has potential applications in the field of monitoring disease therapy, detecting comprehensive food safety hazards and even life in hostile environment.
Highlights
The emergence of antibiotics therapy has led to a medical revolution in infectious disease treatment since the early twentieth century
graphene quantum dots (GQDs) provides a new method for bacteria response to antibiotics investigation
Two platinum electrodes membrane and their response to antibiotics were recorded by the sensing system
Summary
The emergence of antibiotics therapy has led to a medical revolution in infectious disease treatment since the early twentieth century. Materials 2017, 10, 603 in the morbidity and mortality associated with infectious diseases [1]. Such advances in infectious disease treatment boost the applications of antibiotics. The abuse of antibiotics for nonbacterial infections in recent years has resulted in multidrug-resistant bacteria pathogens, which are serious threats to the efficient treatment of diseases with these drugs [2]. Antibiotics are overused as the feed additives for growth promotion in livestock and poultry raising, plant production and aquaculture, having a detrimental effect on human health by direct contact on farms or via the food chain [3,4]. It is very necessary to develop techniques for quick and sensitive detection and characterization of bacterial response to antibiotics in the management of infections and food safety
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