Abstract
Wheat (Triticum aestivum L.) is one of the main crops for human nutrition. The genetic variability of grown wheat has been reduced by modern agronomic practices, which inturn prompted the importance of search for species that could be useful as a genepool for the improving of flour quality for human consumption or for other industrial uses. Therefore, the aim of this study was to analyze the genetic diversity among 24 European wheat genotypes based on Random Amplified Polymorphism (RAPD) markers. A total of 29 DNA fragments were amplified with an average 4.83 polymorphic fragments per primer. The primer producing the most polymorphic fragments was SIGMA-D-P, where 7 polymorphic amplification products were detected. The lowest number of amplified fragments (3) was detected by using the primer OPB-08. The size of amplified products varied between 300 bp (OPE-07) to 3000 bp (SIGMA-D-P). The diversity index (DI) of the applied RAPD markers ranged from 0.528 (OPB-07) to 0.809 (SIGMA-D-P) with an average of 0.721. The polymorphism information content (PIC) of the markers varied from 0.469 (OPB-07) to 0.798 (SIGMA-D-P) with an average 0.692. Probability of identity (PI) was low ranged from 0.009 (SIGMA-D-P) to 0.165 (OPB-07) with an avarage 0.043. The dendrogram based on hierarchical cluster analysis using UPGMA algorithm was prepared. Within the dendrogram was separated the unique genotype Insegrain (FRA) from the rest of 23 genotypes which were further subdivided into two subclusters. In the first subclaster were grouped 13 genotypes and the second subcluster involved 10 genotypes. The first subcluster also included the genotype Bagou from France, in which were detected novel high - molecular - weight glutenin subunits using SDS-PAGE. Using 6 RAPD markers only two wheat genotypes have not been distinguished. Through that the information about genetic similarity and differences will be helpful to avoid any possibility of elite germplasm becoming genetically uniform.
Highlights
Wheat (Triticum spp.) is a self-pollinating annual plant, belonging to the family Poaceae, tribe Triticeae, genus Triticum
The importance of wheat is mainly due to the fact that its seed can be ground into flour, which form the basic ingredients of bread and other bakery products, as well as pastas, and it presents the main source of nutrients such as proteins, carbohydrates, lipids, fibre and vitamins, to the most of the world population
It is necessary to use a higher number of Random Amplified Polymorphism (RAPD) markers
Summary
Wheat (Triticum spp.) is a self-pollinating annual plant, belonging to the family Poaceae (grasses), tribe Triticeae, genus Triticum. Bread wheat (Triticum aestivum L.) is one of the most important and widely cultivated crops used mainly for human consumption and support in the world. The importance of wheat is mainly due to the fact that its seed can be ground into flour, which form the basic ingredients of bread and other bakery products, as well as pastas, and it presents the main source of nutrients such as proteins, carbohydrates, lipids, fibre and vitamins, to the most of the world population. 734.8 million tons of wheat is produced annually on 247 million hectare of the total cultivated land in the world and supports nearly of the worlds population (http://www.fao.org/worldfoodsituation/csdb/en/)
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