Molecular variability of oat based on gene specific markers

Želmí­Ra Balážová,Martin Vivodí­K,Zdenka Gálová,Lenka Petrovičová,Radomí­Ra Hornyák Gregáňová

doi:10.5219/774

ŽelmíRa Balážová, Martin VivodíK + Show 3 more

Open Access

https://doi.org/10.5219/774

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Abstract

Oat (Avena sativa L.) is a grass planted as a cereal crop. Cultivation of oat is increasing in the recent years because of its good nutrition value. The aim of our study was to analyze genetic variability of oat accessions based on SCoT markers. Eighteen primers were used to study polymorfism of 8 oat genotypes. All 18 primers produced polymorphic and reproducible data. Altogether 153 different fragments were amplified of which 67 were polymorphic with an average number of 3.72 polymorphic fragments per genotype. The number of polymorphic fragments ranged from one (SCoT9, SCoT62) to nine (SCoT40). The percentage of polymorphic bands ranged from 14.29% (SCoT9) to 60% (SCoT59) with an average of 41.62%. Genetic polymorphism was characterized based on diversity index (DI), probability of identity (PI) and polymorphic information content (PIC). The diversity index of the tested SCoT markers ranged from 0 (SCoT9, SCoT62) to 0.878 (SCoT40) with an average of 0.574. The polymorphic information content ranged from 0 (SCoT9, SCoT62) to 0.876 (SCoT40) with an average of 0.524. Dendrogram based on hierarchical cluster analysis using UPGMA algorithm grouped genotypes into two main clusters. Two genotypes, Taiko and Vok were genetically the closest. Results showed the utility of SCoT markers for estimation of genetic diversity of oat genotypes leading to genotype identification.

Highlights

Cereals belong to a group of key foods of plant production
start codon-targeted (SCoT) marker system had initially been validated in the model species rice (Oryza sativa) (Collard and Mackill 2009)
Polymerase chain reaction (PCR) amplifications using 18 SCoT primers produced total 153 DNA fragments that could be scored in all genotypes

Summary

Introduction

Cereals belong to a group of key foods of plant production. Oat together with corn and barley is the most used for feed but for human nutrition is used only a little. Cultivated oats are hexaploid cereals belonging to the genus Avena L., which is found worldwide in almost all agricultural environments. Oats have been receiving increasing interest as human food, mainly because the cereal could be suitable for consumptions by celiac patients (Gálová et al, 2012). In the Nordic countries and Northern Europe it became a wellestablished crop both for food and feed. Oat belongs to alternative cereals which are used mainly as a supplement to traditional species of cereals (Daou and Zhang, 2012)

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