Abstract
A chromatographic method permitting fast and complete separation of all nonphospholipid fractions in rat blood and liver except for 1,2-diglycerides from cholesterol, and 1- from 2-monoglycerides is described. The level of radioactivity and the ratio 14C/ 3H were determined in partial glycerides and free fatty acids from blood and liver and in liver phospholipids at short time intervals after the intravenous injection into carbohydrate-fed rats of chyle containing triglycerides labeled with [ 14C]glycerol and [ 3H]palmitic acid. The ratios 14C/ 3H in blood partial glycerides showed that they were not formed by breakdown of chyle triglycerides in the circulating blood, but were probably original constituents of the chyle. The sum of 3H radioactivity in partial glycerides and free fatty acids in the liver never exceeded one tenth of the total 3H radioactivity in this organ. The ratios 14C/ 3H in the labeled liver partial glycerides indicated that these were mainly formed during the resynthesis of triglycerides and phospholipids. There was a progressive rise of fatty acid radioactivity in the liver phospholipids. Only a small amount of 14C radioactivity was found in these—slightly relatively more in the phosphatidyl ethanolamine than in the phosphatidyl choline fraction.
Published Version
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