Abstract
The low extent of the cross-reactions due to the main circulation steroids indicates that radioimmunoassay by the new kit can be directly performed on dried crude extracts of plasma. A radioimmunoassay for the determination of plasma aldosterone is described simply. 1) extraction of aldosterone from 0.5 ml of plasma 2) incubation of the reaction mixture for 20 minutes at 37℃ and for 2 hours at 4℃ 3) absorption of free aldosterone on charcoal-dextran 4) centrifugation at 4℃ and counting of the supernatant However, in a pregnant woman or a patient treated with steroid-hormone, the purification is necessary to be achieved by paper (or column) chromatography. The trial produced paper chromatography box is very useful for these cases.
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