Abstract

The development of gas-liquid chromatography has made possible the rapid analysis of the volatile substances. In the present study, the technique was utilized for the analysis of carbonyls in the oxidized calamary oil methyl ester. The chromatographic separations were carried out on Hitachi KGL-2 gas-chromatograph using silicon grease as a stationary liquid phase because of its thermal stability. Plots of the logarithm of the retention time against the number of carbon atom in authentic carbonyls gave straight lines (Fig. 1, 2). Results shown in Fig. 1, 2 clearly indicate that carbonyls whose numbers of carbon are 3-6 and 7-12 can be analyzed at 90°C and 150°C respectively. Two kinds of samples, ether extract of the steam distillate of the oxidized calamary oil methyl ester and ether extract of the steam distillate treated with 2, 4-dinitrophenylhydrazine solution in which carbonyls were converted to the unvolatile hydrazones were separated at 90°C and 150°C. The elution diagrams illustrated in Fig. 3, 4 demonstrate the successful separation and identification of propanal, butanal, 2-butenal, pentanal hexanal, heptanal, 2-heptenal, 2-octenal, nonanal, and 2, 4-undecadienal (Table 1). In general the results agreed with those obtained by the combined use of column and paper chromatography except for separation of propanal, which indicated the superior accuracy of gas-chromatography. Unsaturated carbonyls identified by gas-liquid chromatography did not reveal any difference from those by paper and column chromatography, and a principal volatile carbonyl component in the oxidized methyl ester was 2-octenal.

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.