Abstract
The main goal of present research is determining the antibacterial power of zinc oxide nanoparticles (ZnO-NPs) synthesized by submerged electrodischarge erosion (SEDE). Therefore, SEDE system as a simple, one-step and eco-friendly route, was employed for producing colloidal ZnO-NPs inside deionized water (DW). The structural composition, morphology and optical property of prepared nanoparticles were characterized and confirmed by x-ray diffraction (XRD) technique, Fourier transform infrared spectroscopy (FTIR), Dynamic light scattering (DLS) analysis, Electron microscopy (TEM and FESEM with EDS) and UV-Visible absorbance spectroscopy. The fabricated ZnO-NPs were appeared in agglomerated semi spherical shapes having a narrow log-normal size distribution (12–22 nm) with an average diameter of 15 nm. From the XRD, the final nanoparticles are composed by a mixture of single crystallites and disordered ZnO. The optical data disclosed the presence of plasmon peak at 354 nm and the optical band gap was found to be 3.65 eV at room temperature accompanied by blueshift. Besides, two standard in vitro antibacterial tests were performed against gram (+ve) Staphylococcus aureus (S. aureus) and gram (−ve) Escherichia coli (E. coli) cells. Surprisingly, synthesized NPs illustrated an incredible antibacterial activity, so that low concentrations of 50 and 100 (μg/ml) are strong enough to complete mortality of S. aureus and E. coli microorganisms respectively. Also, it was demonstrated that the antibacterial response of SEDE grown ZnO nanoparticles, is stronger on gram (+ve) S. aureus cells. An explanation of the high potent antibacterial activity was proposed taking into account the ZnO-NPs structure as a result of peculiar synthesis procedure.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call