Abstract
This paper presents a high performance liquid chromatography (HPLC) method for the determination of riboflavin and free aromatic amino acids. To determine riboflavin, the method includes an acid hydrolysis (HCl 0.1 N) followed by an enzymatic digestion and a protein precipitation with trichloroacetic acid. An analytical column, Chrom SEP SS C18 5 µm (4.6 mm × 150 mm) was used with an isocratic flow using a mobile phase consisting of 5 mM ammonium acetate-methanol (72:28, vv−1), at a flow rate of 1.0 mL min−1 to 28°C. The method showed adequate linearity, repeatability, reproducibility, accuracy, and limits of detection. The method was used to quantify the cited analytes in three different forms of hydrolysate obtained from fermented shrimp remnants.
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More From: Journal of Liquid Chromatography & Related Technologies
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