Abstract
A rapid and simple high-performance liquid chromatographic (HPLC) method with amperometric detection has been developed for the quantitation of labetalol in urine. The chromatography was performed at 30°C using a reversed-phase column with a base deactivated silica stationary support and an alkylamide bonded phase (Supelcosil ABZ+Plus). A 5 m M acetate buffer (pH 4.5)–acetonitrile (70:30, v/v) mixture was employed as the mobile phase, pumped at a flow-rate of 1 ml/min. Sample preparation was carried out using a simple solid-phase extraction (SPE) procedure, and recoveries higher than 85% were achieved. The method was found to be accurate, precise (R.S.D lower than 8%), and sensitive enough (experimental quantitation limit of 20 ng/ml, detection limit 10 ng/ml) to be applied to doping analysis and pharmacokinetic studies in human urine. The method was applied to the determination of labetalol in pharmaceutical formulations and urine samples obtained from a healthy volunteer after the ingestion of a therapeutic dose of the drug, and the results obtained were in agreement with the pharmacokinetic data.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
More From: Journal of Chromatography B: Biomedical Sciences and Applications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.