Abstract
A rapid and simple high performance liquid chromatographic (HPLC) method with amperometric detection has been developed for the quantitative determination of oxprenolol in human urine and pharmaceuticals. The chromatographic method was performed at (25 ± 0.2)°C on a reversed-phase column (Supelcosil ABZ + Plus) with a mobile phase of acetonitrile-water (30:70, v/v) containing 4 mM acetate buffer pH = 4.4 and with a flow rate of 1 mL/min. The amperometric detector equipped with a glassy carbon electrode was operated at +1300 mV versus Ag/AgCl in direct current mode. A simple solid phase (SPE) extraction method was used as clean-up procedure, obtaining recoveries greater than 90% for spiked urine samples. The method was found to be accurate, precise, and sensitive enough to determine free oxprenolol in human urine samples, which would allow its application to doping analysis. The method developed allowed the analysis of urine samples obtained from a patient under treatment with oxprenolol, and to its determination in the pharmaceutical formulation Transitensin (oxprenolol 80 mg + chlortalidone 10 mg).
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