Abstract
A method is described for the quantitative determination of amino acids on filter paper. The staining with Moor and Stein reagent is done in two stages, the first staing being done on the paper directly and the second in a glass tube with the eluted amino acid. As the method is a very sensitive one, different means of control are pointed out. As a result five quantitative determinations of the amino acid composition of normal human albumin are stated. The method should be useful for those already well acquainted with two-dimensional chromatography and where the available quantity of protein material is small.
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