Abstract
Based on the microscopic Beer-Lambert law, two practical time-integrated spectroscopy (TIS) methods, called dual-wavelength spectroscopy method, and dual-wavelength and dual-site spectroscopy method, are described to determine the absolute concentration of an absorber in variously shaped turbid media. We demonstrate, for the first time, the validity of the TIS methods by means of experiments in which the absolute concentrations of an absorber in a tissue-like phantom were determined with errors less than several percent. The advantages and disadvantages of both methods are also discussed.
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