Abstract
Echinacea purpurea plants showing yellowing/reddening and phyllody symptoms were observed in southern Bohemia. Transmission electron microscopy observations showed phytoplasmas in sieve cells of symptomatic plants, but not in healthy ones. Direct and nested polymerase chain reaction with phytoplasma specific primers and subsequent restriction fragment length polymorphism analyses of 16S rDNA as well as sequence analyses of the 16S–23S ribosomal operon (1789 bp) enabled the classification of the detected phytoplasmas in the aster yellows group, ribosomal subgroup 16SrI-C. Identical analyses of the transcription factor tuf gene and the ribosomal protein rpl22, rps3 genes were used for further classification, and revealed the affiliations of the phytoplasmas to the tufIC and rpIC subgroups, respectively. The presence of clover phyllody phytoplasmas in purple coneflowers is a new record for this species.
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