Abstract
Gyroxin, a non-lethal toxin from Crotalus durissus terrificus venom was isolated by a procedure involving two chromatographies on Sephadex G-75 at pH 4·0; ion exchange chromatography on CM-Sephadex C-50 at pH 3·5 and chromatography on Sephadex G-75 at pH 4·0. The estimated purification of gyroxin was 70–80 fold and the yield in the active fraction was 0·8–1·0% with respect to the crude venom sample. Purified gyroxin did not show contamination by lethal components or enzymes present in the venom and behaved as a homogeneous component on SDS-polyacrylamide gel-electrophoresis and immunoelectrophoresis, with a mol. wt of 33,000–34,000. The stability of the purified gyroxin was pH-dependent, being maximal at pH 4·0; is not affected by freezing and thawing or by treatment at 40°C for 15 min. In spite of the fact that gyroxin is not a TAME-esterase nor requires a TAME-esterase for its activity, it is inactivated by TACK (a specific, active site-directed inhibitor of TAME-esterases) at concentrations slightly higher than those which resulted in complete inactivation of TAME-esterases.
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