First report of aflatoxin and ochratoxin contamination in ginger collected from different agroclimatic zones from Punjab, Pakistan

Abstract

Ginger, a fresh rhizome, an economically important spice with extensive nutraceutical activities finds itself in vegetable and therapeutic market. Aflatoxins (AFB1, AFB2, AFG1 and AFG2) along with ochratoxin A (OTA) are the most significant and the most toxic form of mycotoxins which are produced by various fungi. This study was initiated to assess the contamination of AFs and OTA in raw and dried ginger products, collected from different agro-climatic zones in Punjab, Pakistan employing the high performance liquid chromatography. We found all (raw ginger samples commercial ginger powders) samples contaminated with AFB1 (range: 29.88-1060.12μg/kg). AFB2 contamination was much lower (range: 0 to 17.54μg/kg). Variable contamination of AFG1 was also observed (range: 0 to 170.58μg/kg) whereas AFG2 contamination was found in only three (range: 0 to 21.88 μg/kg) out of 19 raw ginger samples. OTA contamination ranged from 0.05-3.42μg/kg. Ginger samples from lower altitudes (<1000 m) were more contaminated with AFB1 sub type mycotoxin. Keeping in view that the toxicity of AFs is in the order of B1>G1> B2>G2, it was alarming to find that 100% of the samples were contaminated with AFB1 way beyond the permissible limits. Our very first report about the contamination of ginger with AFs presents a grave health issue because of wide use of ginger. We conclude that ginger production in Pakistan needs to be carefully crafted and due diligence is needed during ginger cultivation, harvest and post-harvest operations because the amount of aflatoxins detected in this study are very much above the permissible limits. In this regard, ginger storage in cooler environments such as refrigerator should be encouraged to contain the AFs proliferation.