Abstract
Isolation of pure sphingomyelin from human plasma on a preparative scale is described. Analyses of fatty acids by gas-liquid chromatography on two polar polyester columns indicate the presence of all even- and odd-carbon, saturated fatty acids from C14 to C25 and most of the corresponding monoenoic acids as well. Palmitic acid is the predominant component; seven of the acids constitute over 90% of the total fraction. A previously unassigned trace constituent of the fatty acid fraction has been tentatively identified as a C24 dienoic acid on the basis of relative retention times, microhydrogenation, and reaction with mercuric acetate.
Highlights
Isolation of pure sphingomyelin from human plasma on a preparative scale is described
Of the polar lipids that are constituents of describes the preparative isolation of sphingomyelin serum lipoproteins, the sphingolipids are perhaps the from human plasma, determination of fatty acid :base : least well characterized
The methods used for the myelin fatty acids in tissues and fluids containing preparation of sphingomyelin follow those of Hanahan low concentrations of sphingolipids
Summary
Isolation of pure sphingomyelin from human plasma on a preparative scale is described. Of the polar lipids that are constituents of describes the preparative isolation of sphingomyelin serum lipoproteins, the sphingolipids are perhaps the from human plasma, determination of fatty acid :base : least well characterized. The methods used for the myelin fatty acids in tissues and fluids containing preparation of sphingomyelin follow those of Hanahan low concentrations of sphingolipids.
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