Abstract
Quorum sensing (QS) is a bacterial cell-to-cell communication system controlling QS-mediated genes which is synchronized with the population density. The regulation of specific gene activity is dependent on the signaling molecules produced, namely N-acyl homoserine lactones (AHLs). We report here the identification and characterization of AHLs produced by bacterial strain ND07 isolated from a Malaysian fresh water sample. Molecular identification showed that strain ND07 is clustered closely to Pseudomonas cremoricolorata. Spent culture supernatant extract of P. cremoricolorata strain ND07 activated the AHL biosensor Chromobacterium violaceum CV026. Using high resolution triple quadrupole liquid chromatography-mass spectrometry, it was confirmed that P. cremoricolorata strain ND07 produced N-octanoyl-l-homoserine lactone (C8-HSL) and N-decanoyl-l-homoserine lactone (C10-HSL). To the best of our knowledge, this is the first documentation on the production of C10-HSL in P. cremoricolorata strain ND07.
Highlights
Quorum sensing (QS) is a term coiled by Fuqua and co-workers referring to a bacterial cell-to-cell communication system used as a tool to control their physiological processes [1]
It is the aim of this study to acquire isolates, and study the acyl homoserine lactones (AHLs) production by Pseudomonas cremoricolorata strain ND07
An AHL biosensor, namely Chromobacterium violaceum CV026, was used to detect the presence of short chain AHLs [16] whereby CV026 cells will respond by forming a purple pigmentation [14,16]
Summary
Quorum sensing (QS) is a term coiled by Fuqua and co-workers referring to a bacterial cell-to-cell communication system used as a tool to control their physiological processes [1]. Once the production of AHL has exceeded the threshold level, the AHL/LuxR complex will be in an active form and be able to regulate QS genes. This will allow the QS bacteria to synchronize the gene regulation in a cell density-dependent manner allowing group behaviors in unison. Fresh water could be a potential environmental reservoir for various bacteria [11] and it raised our interest in isolating bacteria that possess QS properties from fresh water It is the aim of this study to acquire isolates, and study the AHL production by Pseudomonas cremoricolorata strain ND07
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
