Abstract
In the bacteria kingdom, quorum sensing (QS) is a cell-to-cell communication that relies on the production of and response to specific signaling molecules. In proteobacteria, N-acylhomoserine lactones (AHLs) are the well-studied signaling molecules. The present study aimed to characterize the production of AHL of a bacterial strain A9 isolated from a Malaysian tropical soil. Strain A9 was identified as Burkholderia sp. using matrix-assisted laser desorption ionization-time-of-flight mass spectrometry and 16S rDNA nucleotide sequence analysis. AHL production by A9 was detected with two biosensors, namely Chromobacterium violaceum CV026 and Escherichia coli [pSB401]. Thin layer chromatography results showed N–hexanoylhomoserine lactone (C6-HSL) and N–octanoylhomoserine lactone (C8-HSL) production. Unequivocal identification of C6-HSL and C8-HSL was achieved by high resolution triple quadrupole liquid chromatography-mass spectrometry analysis. We have demonstrated that Burkholderia sp. strain A9 produces AHLs that are known to be produced by other Burkholderia spp. with CepI/CepR homologs.
Highlights
Quorum sensing (QS) refers to bacterial communication via signaling molecules called autoinducers (AIs) [1]
In this paper we present the QS activity of Burkholderia sp. strain A9 isolated from a Malaysian tropical soil
At each interval of 48 h enrichment cycle, an aliquot of KGm was streaked on diluted LB agar (LBA) to observe bacterial growth
Summary
Quorum sensing (QS) refers to bacterial communication via signaling molecules called autoinducers (AIs) [1]. QS controls collective behavior such as biofilm formation, production of virulence, and antibiotic production [3]. Pathogenic bacteria rely heavily on QS systems to control the expression of genes required for virulence in order to escape the immune response for successful infection [4]. Members of Burkholderia are free-living microorganisms found in diverse environments such as soil, water (including sea water), the rhizospheres of plants, humans, various animal species, and the hospital environment [6]. Burkholderia strains are promising candidates for biotechnological applications such as biological control, bioremediation, atmospheric nitrogen fixation, and plant growth stimulation [7,8]. Most of these strains belong to species of the B. cepacia complex (Bcc) which have long been recognized as important human pathogens causing serious infections in the lungs of cystic fibrosis patients. N-acylhomoserine lactones (AHLs) produced by strain A9 were confirmed by high resolution triple quadrupole LC/MS analysis
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