Abstract
In many species of bacteria, the quorum sensing mechanism is used as a unique communication system which allows them to regulate gene expression and behavior in accordance with their population density. N-Acylhomoserine lactones (AHLs) are known as diffusible autoinducer molecules involved in this communication network. This finding aimed to characterize the production of AHL of a bacterial strain ND04 isolated from a Malaysian waterfall. Strain ND04 was identified as Kluyvera sp. as confirmed by molecular analysis of its 16S ribosomal RNA gene sequence. Kluyvera sp. is closely related to the Enterobacteriaceae family. Chromobacterium violaceum CV026 was used as a biosensor to detect the production of AHL by strain ND04. High resolution triple quadrupole liquid chromatography-mass spectrometry analysis of strain ND04 showed our isolate produced two AHLs which are N-(3-oxohexanoyl)homoserine lactone (3-oxo-C6 HSL) and N-3-oxo-octanoyl-L-homoserine lactone (3-oxo-C8 HSL).
Highlights
Quorum sensing (QS) is a cell-to-cell communication mechanism used by many species of bacteria [1,2] in order to measure the density of their own population within their environment and to regulate their gene expression and behavior [3]
We aimed to identify the Acylhomoserine lactones (AHLs)(s) produced by Kluyvera sp. strain ND04 isolated from a Malaysian waterfall
Chromobacterium violaceum CV026 was used as an AHL biosensor which detect the presence of exogenous short chain AHLs ranging from four to eight carbons [18]
Summary
Quorum sensing (QS) is a cell-to-cell communication mechanism used by many species of bacteria [1,2] in order to measure the density of their own population within their environment and to regulate their gene expression and behavior [3]. The biosynthesis of specific signaling molecules, acyl-homoserine lactones (AHLs), known as autoinducers, is regulated by the LuxI-like proteins. LuxR-like protein is the receptor which will bind cognately to the autoinducers when the signal reaches a critical threshold level [8]. These LuxR-autoinducer complexes regulate gene transcription [9]. We have isolated from a Malaysian waterfall the strain ND04 that shows QS properties. It has been identified as Kluyvera sp. We aimed to identify the AHL(s) produced by Kluyvera sp. strain ND04 isolated from a Malaysian waterfall
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