Abstract
Acylation of α-trypsin and purified fractions of stem-bromelain by substituted phenyl ester substrates has a low sensitivity to Hammett σ consistent with the involvement of an electrophilic interaction with the ether oxygen. General-base assisted thiol attack is shown to be a minor route in acylation of thiol proteases. Stem bromelain's heterogeneity is confirmed and a fractionation procedure is reported for isolation of macro-quantities of the constituent enzymes.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of the Chemical Society, Perkin Transactions 2
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
