Protective effects of heparin on early septic shock of intestinal mucosal injury in dogs

Abstract

Objective Determination of plasma markers of D-lactic acid and I-FABP and analyzed to verify the early septic shock in dogs and intestinal fatty acid binding protein expression levels of D-lactate changes and improvements to septic shock heparin early jejunal villus microcirculation in dogs and intestinal fatty acid binding protein and the expression of D-lactic acid levels, confirmed the protective effect of heparin on intestinal mucosal injury. Methods Beagles early septic shock by intravenous injection of LPS Methods. The 40 animals were randomly random number divided into control group, LPS group, the basic treatment group, heparin group. Stable after 1 hour, i. e. the point T0, to modeling LPS 1 hour, i. e. at T1, after 5 hours was observed sequential T2, T3, T4, T5, T6 point. Observed at the corresponding time points (T1, T3, T6 time points) canine blood collected by 1 500 rpm, 15 minutes after centrifugation, the plasma to the EP tube into aliquots stored at -80 ℃, measured by ELISA intestinal fatty acid binding protein (IFABP) and D-lactate (D-lac). Results heparin group IFABP (21.6±2.3) pg / mL LPS comparison group IFABP (100.48±8.42) pg / mL will be significantly decreased, with statistical significance, P 0.05, on the basis of adequate volume resuscitation on heparin therapy, I-FABP expression levels can be significantly reduced. Conclusions The presence of septic shock early intestinal mucosal microcirculation and intestinal mucosa. Under the premise of its full capacity of recovery, heparin early septic shock in dogs with intestinal mucosal microcirculation improvement, thereby protecting the intestinal mucosal barrier function. Key words: Sepsis / Septic shock; Heparin; Intestinal mucosa; Microcirculation; Lipopolysaccharides; Intestinal binding protein; D-lactic acid; Orthogonal polarization spectral imager