Protective effect of low level laser irradiation on liver ischemia-reperfusion injury in rats

Abstract

Objective To investigate the protective effect of low level laser irradiation on liver ischemia-reperfusion injury (HIRI) in rats. Methods Forty-five health male Sprague-Dawley rats were randomly divided into three groups: Sham group, ischemia-reperfusion group (Control group) and 1ow level laser irradiation group (LLLI group). After the hepatic inflow was occluded for 30 min and reperfused for 90 min, serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) were determined, and the liver specimen were obtained for the determination of superoxide dismutase (SOD) activities and malondialdehyde (MDA) contents and for microscopic examinations. Results As compared with the Sham group, the levels of ALT [(577.98±134.65) U/L, (233.11±108.23) U/L], AST [(1 213±314.8) U/L, (563.32±289.77) U/L], LDH [(2 985.96±478.88) U/L, (1 086.24±1 068.31) U/L], MDA [(106.87±30.34) U/L, (54.23±20.37) U/L)] in Control group and LLLI group was significantly increased (P<0.05), and the activity of SOD [(33.15±3.51) U/L, (59.11±7.01) U/L] was significantly lower than in Sham group (P<0.05). As compared with the Control group, the levels of ALT, AST, LDH and MDA in LLLI group were significantly reduced (P<0.05), and the activity of SOD was significantly elevated (P<0.05). Conclusion LLLI has a protective effect on rat livers against ischemia-reperfusion injury, which is related with antioxidation. Key words: Low level laser irradiation; Liver ischemia; Reperfusion injury; Superoxide dismutase; Malondialdehyde