Influence of intravenous injected sodium hydrosulfide on fatty liver ischemia-reperfusion injury in rats

Abstract

Objective To investigate whether intravenous injected sodium hydrosulfide (NaHS) could alleviate the fatty liver ischemia-reperfusion injury in rats and the mechanism. Methods 30 healthy male Sprague-Dawley (SD) rats were fed with high fat diet for 4 weeks then divided into five groups randomly, sham operation group (S group), ischemia-reperfusion group (IR group) and different doses of sodium hydrosulfide groups (NaHS 1-3 groups). In S group hepatic portal was only exposed but not ligated. In IR group the left and middle lobes of hepatic portal vein and hepatic artery were clamped for 1 h and then reperfused. NaHS 1-3 groups received intravenous of NaHS 14, 28, 56 μmol/kg 5 min before reperfusion. Blood samples were collected for measuring the level of alanine transaminase (ALT) in serum at 6 h after reperfusion. Liver tissue samples were collected for measurement the content of malondialdehyde (MDA) and the activity of superoxide dismutase (SOD) and pathologic examination. Results The level of ALT in serum of S group, IR group and NaHS 1-3 group were(64±8), (1 393±69), (852±53), (823±42), (807±57) U/L respectively; The content of MDA in liver tissue of S group, IR group and NaHS 1-3 group were (2.15±0.24), (7.97±0.42), (5.26±0.34), (4.95±0.42), (4.77±0.40) nmol/mg prot respectively; the activity of SOD in liver tissue were (421±20), (201±32), (517±27), (601±23), (607±30) U/mg prot respectively. Compared with S group, the level of ALT in serum and MDA content in liver tissue were increased in IR group (t=44.194, P=0.000; t=42.068, P=0.000), and the activity of SOD in liver tissue was decreased in IR group (t=-14.658, P=0.000). Compared with IR group, the level of ALT in serum were decreased in NaHS 1-3 group (t=-11.563, P=0.000; t=-36.415, P=0.000; t=-13.372, P=0.000), and the content MDA in liver tissue were decreased in NaHS 1-3 group (t=-10.375, P=0.000; t=-17.532, P=0.000; t=-12.402, P=0.000), the activity of SOD in liver tissue was increased in NaHS 1-3 group (t=24.087, P=0.000; t=30.451, P=0.000; t=42.542, P=0.000). The pathological damage degree was lighter in NaHS 1-3 group (Suzuki’s score 2.50, 2.33, 2.17) than IR group (Suzuki’s score 3.67) (t=3.873, P=0.012; t=6.325, P=0.001; t=11.608, P=0.000). Conclusion Intravenous injected sodium hydrosulfide can reduce fatty liver ischemia-reperfusion injury in rats through the inhibition of lipid peroxidation. Key words: Sodium hydrosulfide; Liver ischemia; Reperfusion injury; Fatty liver; Lipid peroxidation