Effects of pretreatment with etomidate on the contents of alanine transaminase, aspartate transaminase, malondialdehyde and superoxide dismutase in dogs with hepatic ischemia/reperfusion injury

Abstract

Objective To investigate the effects of pretreatment with etomidate on hepatic injury and the contents of alanine transaminase (ALT),aspartate transaminase (AST) in plasma and malondialdehyde (MDA),superoxide dismutase (SOD) in hepatic tissue during hepatic ischemia/repeffusion injury(HI/RI) in dogs.Methods 20 adult healthy hybrid canines were randomly divided into 4 groups(n=5): Sham group(group S),ischemia/reperfusion group (group I/R),low dosage of Etomidate group(group El,0.6 mg/kg) and high dosage of Etomidate group (group E2,1.5 mg/kg).Canines in all groups were injected Etomidate or NS except group S.The porta hepatis of canines in group S was separated without ligation.The model of HI/RI were established by ligating porta hepatis.The biochemical indicators of ALT and AST in plasma were measured at 4 time points: before blocked,30 min after ischemis,l,2 h after repeffusion.The contents of MDA and activity of SOD in hepatic tissue were also measured.Hepatic tissue was observed with light microscope after operation.Results Compared with group S,group I/R,group E1 and group E2 increased the level of ALT and AST in plasma (P＜0.01).Compared with group I/R,the level of ALT,AST in group E1 and group E2 decreased significantly(P＜0.01),moreover,group E2 decreased more obviously.Reperfusion 2 h: ALT: (2911±89) U/L(group I/R),(863±95) U/L(group E2); AST: (2722±103) U/L(group I/R),(1056±115) U/L(group E2)].Compared with group S,group I/R,group E1 and group E2 increased the level of MDA and group I/R decreased the expression level of SOD,group E1 and group E2 increased the level of SOD in hepatic tissue (P＜0.01).Compared with group I/R,group E1 and group E2 decreased significantly the level of MDA and increased significantly the level of SOD in hepatic tissue (P＜0.01),and group E2 more obviously[MDA:(21.70±-2.01) nmol·mg-1·prot-1 (group I/R),(12.69±1.02) nmol·mg-1·prot-1 (group E2),SOD: (191±12) nmol·mg-1 ·prot-1(group I/R),(634±26) nmol· mg-1·prot-1 (group E2)].Light microscope showed evidently that the pathological changes of hepatic tissue were more slight in group E1 and group E2 than that in group I/R,and group E2 more slight than group El.Conclusions Pretreatment with etomidate has protective effects on hepatic ischemia reperfusion injury,and it is related to the control lipid peroxidation of etomidate.And the protective effects of high dosage of etomidate (1.5 mg/kg) was more reliable. Key words: Etomidate ; Pretreatment ; Hepatic ischemia/reperfusion ; Alanine transaminase ; Aspartate transaminase; Malondialdehyde; Superoxide dismutase