Protective effect of diltiazem, a L-type calcium channel antagonist, on bisphenol A-enhanced hydroxyl radical generation by 1-methyl-4-phenylpyridinium ion in rat striatum

Abstract

The present study examined whether bisphenol A enhanced 1-methyl-4-phenylpyridinium ion (MPP+)-induced hydroxyl radical (·OH) generation in rat striatum using microdialysis technique. These elevations were detected by increases in non-enzymatic formation of 2,3-dihydroxybenzoic acid levels. Bisphenol A significantly enhanced MPP+-induced ·OH generation. Further, the effect of diltiazem, a L-type Ca2+ channel antagonist, on bisphenol A (10 μM) and MPP+ (5 mM)-induced ·OH generation were studied. Ddiltiazem (100 μM) significantly suppressed bisphenol A and MPP+-induced ·OH generation. The results indicate bisphenol A augmented ·OH generation, and diltiazem may have preventive effect on bisphenol A and MPP+-induced ·OH generation.