Abstract
In order to determine whether or not prophage induction leads to excision of the prophage DNA from the host chromosomal DNA, the level of linkage between two markers on each side of the prophage φ80 attachment site, Trp and UDPG, was examined by P1 transduction. After induction of i λ h +80 prophage, the cotransduction frequency of these two markers increased from about 10% to approximately one-third of that of the nonlysogenic control. Induction of sus N mutants resulted in 110 increase in cotransduction frequency, whereas induction of sus O and sus P mutants gave an increase in cotransduction frequency to about half the level of the nonlysogenic control. In the presence of chloramphenicol, the increase in cotransduction frequency was not observed. From these results it has been concluded that prophage excision and sealing of the host chromosome occur as the result of induction and that this process requires protein but not prophage DNA synthesis.
Published Version
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