Abstract
Summary The role of the carboxy-terminal domain of the SV40 large T antigen in transformation, has been assessed by isolating mutants modified in this region. In order to closely parallel the polyoma hr-t mutant's genotype, the mutants were isolated in small-t-antigen-defective strains. The target was the last 30 residues whose role in the late phase of the productive infection can be formally identified with polyoma hr-t gene activity. We have isolated a frameshift mutant, called dl2113 lacking the last 26 residues. This mutation which is lethal for virus production does not impair viral DNA replication nor does it impair the transforming capacity of the virus.
Published Version
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