Properties and potential applications of bioconjugates of R-phycoerythrin with Ag° or CdS nanoparticle synthesized in its tunnel cavity: A review

Abstract

Green synthesis is a promising method for the preparation of nanoparticles (NPs) due to its simplicity, low cost, low toxicity, and environmental friendliness. Biosynthesized NPs exhibit multifunctional activity, good biocompatibility, and higher anticancer and antibacterial activity compared to chemically synthesized NPs. R-phycoerythrin, a photosynthetic light-harvesting pigment of protein nature (M.w. 290 kDa), is an attractive platform for the synthesis of small sizes NPs due to its structural features, non-toxicity, water solubility. Photosensitive bioconjugates of R-phycoerythrin with NPs were prepared by synthesizing Ag° and CdS NPs in tunnel cavities of R-phycoerythrin (3.5 × 6.0 nm) isolated from the red seaweed Callithamnion rubosum. The review is devoted to the physical processes and chemical reactions that occur in the native protein macromolecule of a complex structure during the synthesis of a NP in its cavity. The influence of Ago and CdS NPs on the electronic processes caused by the absorption of photons, leading to reversible and irreversible changes in R-phycoerythrin has been analyzed. Properties of R-phycoerythrin bioconjugates Ag° and CdS with NPs combined with the literature data suggest potential applications of Ag°⋅PE and CdS⋅PE bioconjugates for cancer diagnosis, treatment, and monitoring as well as for realizing theranostic strategy in the future. The use of these bioconjugates in anticancer therapy may have synergistic effects since both R-phycoerythrin and NPs induce cancer cell death.