Abstract
This chapter presents an investigation of certain aspects of transfer of electronic excitation energy and of primary and associated reactions of a system. The linear relationship between the reciprocal of the emission and the fraction of emission quenchers indicates that the excitation energy can be transferred over a matrix of many units. If the emission consists largely of fluorescence and if the transfer is so rapid that the excitation energy visits a representative part of the matrix, then this fluorescence decays exponentially with a decay time proportional to the fluorescence yield. In the investigation, an analysis of the time curves of the decay measured by means of a single-photon counting technique showed that for Chlorella vulgaris and other species, the decays in the nanosecond region were non- or multi-exponential, which was attributed to limited transfer between units or pigment–protein complexes.
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