Promotion Saudi-Customs Using Coleus forskohlii L., Via In-Vitro Water-extract on Human-pathogenic-bacteria, Ranyah-region

Sherifa Mostafa M Sabra,Afaf Bushara M Ismail

doi:10.31579/2766-2314/048

Abstract

Background: Coleus forskohlii plant called in KSA (Al-Shar or Aspane Zattar), it discovery nearly at western south area, KSA. It contained flavonoids, alkaloids, tannins, terpenoids, steroids, saponins, and sugars. The essential oil had crucial result against human-pathogenic-bacteria as Bacillus Spp., Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. The aim: It was the first time in Ranyah-region, KSA for proving and supporting the ancient Saudi-customs using Coleus forskohlii plant boiled-water-extract to protect person-health from human-bacterial-diseases and to consider as medicinal plant that easy getting, cheep and grows mostly in the southwestern region, KSA. Materials and Methods: Coleus forskohlii plant were collected from Ranyah-region, KSA, to powder obtaining. The identified human-pathogenic-bacteria were obtained via the laboratory from diagnosed infected human cases. The bacterial suspension were added to boiled-water-extract, were followed up the bacterial growth. Results: The average dry cell weight estimation showed Pseudomonas aeruginosa stayed for 50 hour, Klebsiella pneumoniae 45 hour and Escherichia coli 40 hour. Staphylococcous aureus remained alive for 35 hour, Bacillus Spp., 30 hour. The average membrane filter estimation was found Escherichia coli did not give a large number of colonies at 40 hours, Klebsiella pneumoniae stopped producing colonies till 45 hours and Pseudomonas aeruginosa persisted until 50 hour. Conclusions: It was concluded that the Coleus forskohlii plant boiled-water-extract eliminated human-pathogenic-bacteria, as the ancient Saudi-customs were based on its use for the person-health and its adoption as a medicinal plant. Recommendations: It was recommend that the plant considering as a Saudi medicinal plant and using daily to maintain the person-health.

Highlights

Coleus forskohlii plant is a public earthy medicinal plant from Coleus spp., discovery in Asia, Africa, Australia, and Pacific Islands [1]
The average dry cell weight estimation showed Pseudomonas aeruginosa stayed for 50 hour, Klebsiella pneumoniae 45 hour and Escherichia coli 40 hour
The average membrane filter estimation was found Escherichia coli did not give a large number of colonies at 40 hours, Klebsiella pneumoniae stopped producing colonies till 45 hours and Pseudomonas aeruginosa persisted until 50 hour

Summary

Materials and Methods

Coleus forskohlii plant in Ranyah-region is famous presence in the valley and mountains, this plant was collected at the time of flowering and was graded by the “Botany Department”. The samples were washed with distilled water, was dried for five days, and was milled in a sterile mixer to obtain the powder. The obtained bacteria were sub-cultured onto Mueller-Hinton agar for 24 hour, the colonies were grown in Mueller-Hinton broth as bacterial suspension matching the 0.5 McFarland turbidity standard equal (1.5X108 CFU / mL) [5]. The bacterial suspension 5 ml were added to 22 test tubes for each bacterial strain, the boiled-water-extract 5 ml were added to each test tube. Dry cell weight estimation: The bacterial cells were washed several times with sterile distilled water, the cells were dried and were weighed [7]. The filters were transferred to the MuellerHinton agar, were incubated at 37oC for 24 hour, the colonies growth from the bacteria on the filter’s surface were recorded [8]. The data had recorded and had treated by "Simple Excel Sheet" [9]

Results

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Introduction

Results and Discussion

Conclusion