Prolactin-releasing peptide-containing neurons in the nucleus of the solitary tract activate neurons in the hypothalamic dorsomedial nucleus in response to refeeding of fasted rats

Abstract

Event Abstract Back to Event Prolactin-releasing peptide-containing neurons in the nucleus of the solitary tract activate neurons in the hypothalamic dorsomedial nucleus in response to refeeding of fasted rats Éva Renner1*, Kinga Ibolya1, Szabo Meltzer1, Nela Puskas1, Arpad Dobolyi1, Zsuzsanna Tóth1 and Miklos Palkovits1 1 Department of Anatomy, Histology and Embryology, Semmelweis University, Hungary The regulation of food intake is a hot topic of neuroscience research because it bears increasingly important clinical and social implications. In an attempt to identify brain centers involved in the central regulation of food intake, we examined neurons activated by refeeding of previously fasted rats. A cell group expressing c-Fos in response to refeeding was identified in the ventral subdivision of the hypothalamic dorsomedial nucleus (DMH) by immunocytochemistry and in situ hybridization histochemistry. We also found that the distribution of prolactin-releasing peptide (PrRP)-containing fiber terminals correlated remarkably well with the distribution of activated DMH neurons. To determine whether the PrRP-containing fiber terminals around the activated neurons arise from the hypothalamic or the medullary PrRP-expressing cell group, the medullary-hypothalamic pathway containing the ascending PrRP fibers originating in the nucleus of the solitary tract was transected at the level of the pontine-medullary junction, uni- and bilaterally, and at the level of the caudal hypothalamus. The transection of PrRP-containing fibers was demonstrated by accumulation of immunoreactivities caudal to the transection in fibers and cell bodies. In transected animals, the density of PrRP-immunoreactive fibers decreased dramatically in the DMH. The ipsilateral disappearance of fibers following unilateral transections suggests ipsilateral projections of PrRP neurons. In addition, we also found a marked decrease in the number of DMH neurons expressing c-Fos in response to refeeding following the transection of ascending PrRP fibers. Our findings suggest that PrRP-containing fibers originating in the nucleus of the solitary tract contribute to the activation of DMH neurons during refeeding. Conference: 12th Meeting of the Hungarian Neuroscience Society, Budapest, Hungary, 22 Jan - 24 Jan, 2009. Presentation Type: Poster Presentation Topic: Homeostatic regulatory mechanisms Citation: Renner É, Ibolya K, Meltzer S, Puskas N, Dobolyi A, Tóth Z and Palkovits M (2009). Prolactin-releasing peptide-containing neurons in the nucleus of the solitary tract activate neurons in the hypothalamic dorsomedial nucleus in response to refeeding of fasted rats. Front. Syst. Neurosci. Conference Abstract: 12th Meeting of the Hungarian Neuroscience Society. doi: 10.3389/conf.neuro.01.2009.04.117 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 04 Mar 2009; Published Online: 04 Mar 2009. * Correspondence: Éva Renner, Department of Anatomy, Histology and Embryology, Semmelweis University, Budapest, Hungary, evarenner@yahoo.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Éva Renner Kinga Ibolya Szabo Meltzer Nela Puskas Arpad Dobolyi Zsuzsanna Tóth Miklos Palkovits Google Éva Renner Kinga Ibolya Szabo Meltzer Nela Puskas Arpad Dobolyi Zsuzsanna Tóth Miklos Palkovits Google Scholar Éva Renner Kinga Ibolya Szabo Meltzer Nela Puskas Arpad Dobolyi Zsuzsanna Tóth Miklos Palkovits PubMed Éva Renner Kinga Ibolya Szabo Meltzer Nela Puskas Arpad Dobolyi Zsuzsanna Tóth Miklos Palkovits Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.