Brainstem PrRP- and GLP-1-containing projections to the refeeding activated neurons of the hypothalamic dorsomedial nucleus of fasted rats

Abstract

Event Abstract Back to Event Brainstem PrRP- and GLP-1-containing projections to the refeeding activated neurons of the hypothalamic dorsomedial nucleus of fasted rats Eva Renner1*, Kinga I. Szabó-Meltzer1, Nela Puskas1, Zsuzsanna E. Tóth1, Arpád Dobolyi1 and Miklós Palkovits1 1 Semmelweis University and Hungarian Academy of Sciences, Neuromorphological and Neuroendocrine Research Laboratory, Department Anatomy, Histology and Embryology, Hungary A cell group expressing c-fos in response to refeeding of fasted rats was previously identified in the ventral subdivision of the hypothalamic dorsomedial nucleus (DMH). Here, we report that these cells are also activated after feeding in food-entrained rats. However, Fos does not appear in these cells following the presentation of food without consumption suggesting that satiation and not craving for food may account for the activation. Prolactin-releasing peptide (PrRP)- and glucagon-like peptide-1 (GLP-1)-containing fiber terminals overlap with the distribution of activated neurons in the DMH. PrRP nerve terminals contained tyrosine-hydroxylase (TH) suggesting that they arise in A2 (nucleus of the solitary tract – NTS ) and A1 ( ventrolateral medulla ) noradrenaline neurons where TH and PrRP co-express. GLP-1 neurons are located in non-TH expressing neurons of the NTS suggesting that this part of the medulla may be the source of both PrRP and GLP-1 fibers reaching the DMH. Transections of the medullary-hypothalamic pathway resulting in an accumulation of PrRP- and GLP-1 immunoreactivities caudal to the transections caused the density of PrRP- and GLP-1 fibers decrease dramatically in the DMH. These observations clearly show that PrRP and GLP-1 fibers in the ventral subdivision of the DMH are of medullary – in the case of GLP-1 exlusively- NTS origin. In addition, we detected a marked 74% decrease in the number of Fos-expressing DMH neurons in response to refeeding following transections. In contrast, Fos response was not attenuated following lesion of the arcuate nucleus with neonatal monosodium-glutamate treatment. Our findings suggest that PrRP- and GLP-1-containing solitary-hypothalamic projections contribute to the activation of DMH neurons during refeeding possibly by conveying information on cholecystokinin mediated satiation. This study was supported by OTKA NK72929 grant. Conference: IBRO International Workshop 2010, Pécs, Hungary, 21 Jan - 23 Jan, 2010. Presentation Type: Poster Presentation Topic: Homeostatic and neuroendocrine systems Citation: Renner E, Szabó-Meltzer KI, Puskas N, Tóth ZE, Dobolyi A and Palkovits M (2010). Brainstem PrRP- and GLP-1-containing projections to the refeeding activated neurons of the hypothalamic dorsomedial nucleus of fasted rats. Front. Neurosci. Conference Abstract: IBRO International Workshop 2010. doi: 10.3389/conf.fnins.2010.10.00120 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 28 Apr 2010; Published Online: 28 Apr 2010. * Correspondence: Eva Renner, Semmelweis University and Hungarian Academy of Sciences, Neuromorphological and Neuroendocrine Research Laboratory, Department Anatomy, Histology and Embryology, Budapest, Hungary, evarenner@yahoo.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Eva Renner Kinga I Szabó-Meltzer Nela Puskas Zsuzsanna E Tóth Arpád Dobolyi Miklós Palkovits Google Eva Renner Kinga I Szabó-Meltzer Nela Puskas Zsuzsanna E Tóth Arpád Dobolyi Miklós Palkovits Google Scholar Eva Renner Kinga I Szabó-Meltzer Nela Puskas Zsuzsanna E Tóth Arpád Dobolyi Miklós Palkovits PubMed Eva Renner Kinga I Szabó-Meltzer Nela Puskas Zsuzsanna E Tóth Arpád Dobolyi Miklós Palkovits Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.