Abstract
IFNα-2b as a protein is a cytokine used to treat more than 14 diseases all around the world, the recombinant human IFN-2b was synthesized and a genetic engineering method or recombinant DNA technology. IFNα-2b as a protein with broad biological action, including antiviral and anticancer properties, might be a useful therapeutic protein for a variety of diseases, including hepatitis C and cancer. IFN-α2b sequence was taken from the National Center for Biotechnology Information (NCBI) gene database and optimized before being cloned and produced in the pET28a+ vector. Escherichia coli was considered as a prokaryotic expression system. IFN-α2b expression was carried out in E. coli strains BL21 (DE3) and BL21(DE3) pLysS and E. coli DH5α strain used for cloning. isopropyl-d-thiogalactoside (IPTG) induced protein production in bacteria cells bearing the pET28a+ IFN-α2b construct, followed by sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation of total proteins. Western blotting was used to confirm that IFN-α2b was expressed successfully in E. coli strains BL21 (DE3) and BL21(DE3) pLysS.
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