Abstract
An efficient method for production of plantlets from horseradish hairy roots ( Armoracia rusticana) was developed and the plantlets were encapsulated and used as artificial seeds. Hairy root fragments were obtained by fragmentation of hairy roots in a blender after the root growth. A fragmentation time of 30 s gave the best result on the plantlet formation. Treatment of the hairy roots with naphthaleneacetic acid (NAA, 1.0 mg/ l) prior to fragmentation resulted in a decrease in the time required for hairy root growth. Kinetin supplementation of 0.1 mg/ l after fragmentation (in the plantlet formation stage) resulted in the highest number of plantlets. Furthermore, we found that the kinetin treatment led to a change in the size distribution of plantlets and inhibition of root elongation, which facilitated encapsulation of plantlets. Although the frequency of plantlet development was significantly reduced by encapsulation in 2% calcium alginate beads, dehydration treatment before the encapsulation strongly improved the plantlet development frequency.
Published Version
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