Abstract
Horseradish (Armoracia rusticana) hairy roots were established by transformation with the Ri plasmid T-DNA in Agrobacterium rhizogenes 15834. To elucidate the effects of sugar and osmotic pressure on the differentiation of adventitious shoots in horseradish hairy roots, the hairy-root clone AR450 was cultured in phytohormone-free LS medium containing sucrose, glucose,and fructose under light conditions. Adventitious shoots were formed in the presence of 1.5% (W/V) glucose or 1.5% (W/V) fructose as well as in the presence of 3% (W/V) sucrose. High osmotic pressure inhibited the formation of adventitious shoots in horseradish hairy roots. Adventitious shoots were also formed effectively in liquid LS medium without phytohormones under light conditions.
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