Production of Phytase, Amylase and Cellulase by Aspergillus, Rhizophus and Neurospora on Mixed Rice Straw Powder and Soybean Curd Residue

Abstract

There is consensus that the presence of phytate in poultry diet negatively influence protein and energy utilization in poultry, and these influences could be enhanced by hydrolytic enzymes augmentation. The objective of study was to produce phytase, amylase and cellulase by Aspergillus niger, Rhizopus oryzae and Neurospora sitophila on solid state fermentation. Phytase production ability was first determined on submerge fermentation (SmF) using glucose as the main C-sources and on solid-state fermentation (SSF). The media composition for phytase production were a combination of rice straw powder and soybean curd residue at ratio of 0-100 % w/w. Maximum enzyme activities were observed at 96 h of incubation. SSF produced higher phytase than SmF. Maximum phytase production was achieved by N. sitophila (195.66 U/g) followed by A. niger and R. oryzae. Best media formula was 30:70 w/w of rice straw powder and soybean curd residue, respectively. At the same media formulation, the highest amylase was obtained by A. niger (141.85/g) followed by R. oryzae and N. sitophila. While for cellulase A. niger (92.0 U/g) followed by N. sitophila and R. oryzae. The ability of A. niger, R. oryzae and N. sitophila to produce phytase, amylase and cellulase on mixed substrate containing rice straw powder and soybean curd residue implies that those isolates are potential for hydrolytic production for poultry feed.