Production of Nα-benzyloxycarbonyl- l-aminoadipic acid and Nα-benzyloxycarbonyl- d-aminoadipic acid with Rhodococcus sp. AIU Z-35-1

Abstract

We isolated a new bacterial strain capable of producing N α-benzyloxycarbonyl- l-aminoadipic acid ( N α-Z- l-AAA) and N α-Z- d-AAA by cell reaction. This isolated strain was identified as a member of the genus Rhodoccocus. By this strain, N α-Z- l-AAA and N α-Z- d-AAA were formed from N α-Z- l-lysine and N α-Z- d-lysine via N α-Z- l-aminoadipate-δ-semialdehyde ( N α-Z- l-AASA) and N α-Z- d-AASA, respectively, and a more than 10-fold higher concentration of N α-Z- l-AAA was produced in one-third the reaction time of Aspergillus niger AKU 3302. In addition, approximately 40 mM N α-Z- d-AAA was produced from 50 mM N α-Z- d-lysine. Thus, the production of N α-Z- l-AAA and N α-Z- d-AAA was remarkably improved using the new isolated strain.