BIOCHEMICAL STUDIES ON THE ANTIMICROBIAL EFFECT OF SOME MEDICINAL PLANTS

Abstract

In this study, extracts of leaves of three types of plants were made, which are thyme, lemon grass and rosemary, using three solvents, which are 80% ethanol, 80% acetone and water. The chemical composition of the leaves of these plants was studied and the phenolic compounds were estimated by the HPLC device, as well as evaluating the antimicrobial activity of these extracts at concentrations (50, 100, 200, 400 mg / ml). Its inhibitory effect on pathogenic tuberculosis against three strains of Gram-positive bacteria: Staphylococcus aureus, Bacillus cereus, Streptococcus and three strains of Gram-negative bacteria: Escherichia coli, Salmonella typhi and Shigell specie as well as two Aspergillus flavus, Aspergillus niger . Thyme extracts showed anti-bacterial effects (ethanol, acetone, water) maximum diameters of the area inhibitory against (Escherichia coli, Salmonella typhi and Streptococcus pyogenes, respectively), while recorded the same extracts less countries of the region inhibitory against (Bacillus cereus, Escherichia coli and Staphylococcus aureus, respectively). Thyme extracts showed anti-fungal effects (ethanol, acetone and water) the maximum inhibitory zone diameters against (Aspergillus flavu, Aspergillus nigar and Aspergillus flavu respectively) while the same extracts recorded the lowest inhibitory zone diameters against (Aspergillus nigar, Aspergillus flavu and Aspergillus niger respectively). Lemongrass extracts showed the anti-bacterial effects (ethanol, acetone, water) maximum inhibitory zone diameters against (Escherichia coli, Salmonella typhi, andStaphylococcus aureus respectively) while the same extracts recorded the lowest inhibitory zone diameters against (Staphylococcus aureus, Staphylocacus aureus and Bacillus cereus Respectively). lemongrass extracts showed anti-fungal effects (ethanol, acetone, water) the maximum inhibitory zone diameters against (Aspergillus nigar, Aspergillus flavu and Aspergillus flavu respectively) while the same extracts recorded the lowest inhibitory zone diameters against (Aspergillus flavus , Aspergillus nigar and Aspergillus nigar Respectively ) . Rosemary extracts showed anti-bacterial effects (ethanol, acetone, water) maximum diameters of the area inhibitory against (Escherichia coli, Bacillus cereus and Shigell specie respectively), while recorded the same extracts less countries of the region inhibitory against (Staphylococcus aureus, Staphylococcus aureus, Streptococcus pyogenes, respectively), Thyme extracts showed the anti-fungal effects (ethanol, acetone, water) the maximum inhibitory zone diameters against (Aspergillus flavu, Aspergillus nigar and Aspergillus nigar, respectively) while the same extracts recorded the lowest inhibitory zone diameters against (Aspergillus nigar, Aspergillus flavus and Aspergillus flavus respectively) .