Abstract
Minor ginsenosides (MGs) (include ginsenoside F2, Compound K, PPT, etc), which are generally not produced by ginseng plants naturally, are obtained by deglycosylation of major ginsenosides. However, the conventional processes used to produce deglycosylated ginsenosides focus on the use of intestinal microorganisms for transformation. In this study, an edible and medicinal mushroom Stereum hirsutum JE0512 was screened from 161 β-glucosidase-producing soil microorganisms sourced from wild ginseng using the plate coloration method. Furthermore, JE0512 was used for the production of CK from ginseng extracts (GE) in solid-state fermentation (SSF) using 20 g corn bran as substrate, 4 g GE, and 20% inoculation volume, and the results showed that the highest CK content was 29.13 mg/g. After combining S. hirsutum JE0512 with cellulase (Aspergillus niger), the MGs (F2, CK, and PPT) content increased from 1.66 to 130.79 mg/g in the final products. Our results indicate that the Stereum genus has the potential to biotransform GE into CK and the combination of S. hirsutum JE0512 and cellulase could pave the way for the production of MGs from GE.
Highlights
Ginseng is a perennial herbaceous plant of the genus Panax in the Araliaceae family
Four strains possessed the activity of transforming ginseng extracts (GE) into Compound K (CK) as shown by HPLC analysis (Fig 2C and 2D), with JE0512 showing the highest activity in transforming GE into CK (8.34 mg/g)
The results indicated that solid-state fermentation (SSF) combined with cellulase (A. niger) and S. hirsutum enhances the conversion of ginsenosides into Minor ginsenosides (MGs)
Summary
Ginseng is a perennial herbaceous plant of the genus Panax in the Araliaceae family. Asian (Panax ginseng) and American ginseng (Panax quinquefolius) are the two most well-known species and are both commonly used for their medicinal properties [1]. Ginseng is a famous and valuable medicinal and edible plant; this perennial herb has been used to enhance immunity, reduce fatigue, slow aging, and provide nutrition around the world [2]. Modern pharmacological experiments have shown that ginseng contains a variety of bioactive compounds, including ginsenosides, polysaccharides, phenols, and polyacetylenes [3]. Ginsenosides are considered to be the main components underlying the pharmacology and biological activity of ginseng [2]. As of the time of writing, about 180 ginsenoside derivatives have been identified from different parts of Panax quinquefolius, processed P. ginseng products, and other Panax spp.
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call