Abstract
Production of bioactive peptides from corn gluten meal (CGM) by solid-state fermentation (SSF) with Bacillus subtilis MTCC5480 (BS5480) and the antioxidant capacity in vivo were investigated in the present study. During the SSF process, CGM peptides (CGMP) production reached a maximum (369.4 mg/gdp) when the fermentation conditions were as follows: temperature, 36 °C; initial pH, 6.0; moisture, 46%; inoculation size, 5.8 × 109 spores/g; time, 5 d; peptone, 5 mg/g; and glucose, 10.7 mg/g. In addition, the d-galactose-induced aging rats fed CGMP (molecular weight < 10 kDa) exhibited increased activities of total superoxide dismutase, catalase, glutathione peroxidase and total antioxidant capacity in the serum and liver and a decreased malondialdehyde concentration, which indicated that CGMP had good in vivo antioxidant capacity. Overall, SSF with BS5480 is an effective way for converting CGM into CGMP that may be used as a natrual antioxidant.
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