Abstract
A novel and effective bio-fungicide, Trichoderma harzianum CH1 was isolated earlier and characterized for its potential to prevent infestation of pepper and ginger crops by phytopathogens like Phytophthora spp., Rhizoctonia spp., and Fusarium spp. Response surface methodology based central composite design has been applied with T. harzianum on coffee husk (lab-scale, 10 g of substrate) for optimizing the fermentation parameters for enhancing biomass production. The bioprocess parameters such as temperature, moisture content, carbon sources and ammonium sulphate were optimized. A combination of temperature (30 °C), moisture content (67.5% v/w), jaggery (2% w/v), and ammonium sulphate (2% w/v) was found optimum for maximum production. The biomass yield of 143.24 mg/gram of dried fermented substrate was 2.05 times the yield on the optimized conditions by the one-factor-at-a-time method and 3.64 times of the randomly selected conditions. Enhanced production of T. harzianum has great potential to be developed as substitute of toxic fungicides.
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