Abstract
Antibiotic resistance is a serious problem of present world and development of viable alternative is urgent. The research work was designed to mitigate this problem. Different types of bacterial colony were isolated from skin of 30 healthy human and their antipathogenic activity was tested against 9 pathogens. The isolate showed activity against four pathogens- Klebsiella. pneumoniae subsp. pneumoniae, Klebsiella. pneumoniae subsp. ozaenae, Staphylococcus. aureus and Pseudomonas. aeruginosa was identified as Staphylococcus. warneri. Variation was found in optimization of cultural conditions (incubation period, incubation temperature and pH) for the most potent antipathogenic metabolites production. Staphylococcus. warneri showed most potent antipathogenic activity at pH 6 to pH 9, at an incubation period of 24h to 48h and at an incubation temperature of 37℃. Antipathogenic metabolites were then detected as bacteriocin like substances. Variation was observed with bacteriocin activity against different pathogens and was found most effective against Klebsiella. pneumonia subsp. pneumonia and Klebsiella. pneumonia subsp. ozaenae Samples containing bacteriocin like substances showed heat stability up to 80℃ for 60 minutes and up to pH 8 for Klebsiella. pneumoniae subsp. pneumoniae and Klebsiella. Pneumoniae sub sp. ozaenae. Papain treated cell-free supernatant did not show any bacteriocin activity, suggesting that the substances could be antimicrobial peptides. Solvent extraction of bacteriocin was performed by using chloroform where maximum bacteriocin activity was found in interface layer rather than aqueous and organic layer.
Highlights
Microbes that colonize the human body during birth or shortly thereafter, remaining throughout life, are referred to as normal flora [1]
Staphylococcus. epidermidis, a major constituent of the normal microflora on healthy human skin protect skin by producing antimicrobial peptide Another research work [8] on bacteriocin- like inhibitory substance (BLIS) which is produced by the Streptococcal normal flora of the nasopharynx were found active against pathogens Streptococcus pneumoniae, Streptococcus pyogenes, Haemophilus. influenzae and Moraxella
We have tried to investigate the ability of Universal Journal of Microbiology Research 5(3): 40-48, 2017 normal skin flora to produce antipathogenic metabolites and tried to reveal the fact that whether the metabolites were bacteriocin like substances or not
Summary
Microbes that colonize the human body during birth or shortly thereafter, remaining throughout life, are referred to as normal flora [1]. Human skin is not a rich place for microbes to live This is an environment that prevents the growth of many microorganisms, but a few have adapted to life on our skin [3]. Normal flora produces three types of antimicrobial substances- H2O2, organic acid and Bacteriocin [5]. Epidermidis, a major constituent of the normal microflora on healthy human skin protect skin by producing antimicrobial peptide Another research work [8] on bacteriocin- like inhibitory substance (BLIS) which is produced by the Streptococcal normal flora of the nasopharynx were found active against pathogens Streptococcus pneumoniae, Streptococcus pyogenes, Haemophilus. We have tried to investigate the ability of Universal Journal of Microbiology Research 5(3): 40-48, 2017 normal skin flora to produce antipathogenic metabolites and tried to reveal the fact that whether the metabolites were bacteriocin like substances or not
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