Abstract
Fruit rot disease of strawberry caused by fungus is most economically important disease in Bangladesh, which reduces the production and quality of strawberry at both pre and post-harvest period. Present study was conducted to identify the causal organism of the fruit rot disease of strawberry through classical and molecular techniques and its eco-friendly control measures. A fungal pathogen causing fruit rot was isolated and identified as Botrytis cinerea through classical fungal taxonomy and molecular characterization based on their internal transcribed spacer (ITS) region of ribosomal DNA (rDNA). Sequence analysis showed that 5.8S of rDNA sequences were identical. The reciprocal homologies of the ITS region sequences ranged from 98 to 100%. Experimental results suggested that studied fungus (MH371474.1) was genetically similar with MF521935.1 Botrytis cinerea. Isolated fungus was evaluated on six different culture media and at five different temperature conditions. The optimum mycelial growth of B. cinerea was found on Richard agar medium at 25 ℃ temperature at 7days post inoculation (dpi), while mycelial growth was drastically reduced the temperature 30 ℃ and above. Three Trichoderma species viz., T. ressei, T. harzianum and T. asperellum were used to assess the antagonistic effect on isolated pathogenic fungus in which the mycelial growth of it was inhibited mostly by T. ressei. Experimental results revealed that Trichoderma ressei was the effective antagonistic fungus against Botrytis cinerea for the biological control.
Highlights
Strawberry is a stoloniferous perennial herb its cultivation and environmental conditions are very suitable in Bangladesh
It was characterized by abundant hyaline conidia borne on grey, branching tree-like conidiophores
The results indicated that all the individual species of Botrytis cinerea belongs to single major cluster
Summary
Strawberry is a stoloniferous perennial herb its cultivation and environmental conditions are very suitable in Bangladesh. Isolated from Strawberry Fruit Rot Disease in Bangladesh causing phomopsis leaf blight [5]. Anthracnose disease of strawberry caused by Colletotrichum gloesporioides has been described in Bangladesh [6]. Leaf blight and fruit rot disease of strawberry caused by Curvularia spp. had been discovered in Kurdistan province, Iran, in 2013. Molecular identification of fungi through DNA barcoding has become important part of mycological research. It has recently been accepted as the official primary barcoding marker for fungi. Considering the above facts, the present research has been under taken to isolate and identify of strawberry pathogen using morphological and molecular techniques, to investigate the fungal biology and to evaluate the antagonistic fungal effect against Botrytis cinerea
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