Abstract
Publisher Summary The objectives of this chapter are (i) to construct a hybridization-based genosensor for a 110 bp region of the TNFRSF21 gene (human tumor necrosis factor receptor superfamily, member 21 precursor) on a screen-printed carbon electrode (SPCE) modified with streptavidin which works as immobilization and transduction surface, (ii) to obtain both polymerase chain reaction (PCR) products labelled with FITC and unlabelled PCR products; latter products are labeled with fluorescein ULSs Kit, and (iii) to test these PCR products on the genosensor using alkaline phosphatase as enzymatic label and 3-indoxyl phosphate as enzymatic substrate. The results indicate that when the PCR products are labeled using the FITC labeled 5'-primer the analytical signals are higher and more reproducible than those obtained using the ULS labeling kit. Moreover, the PCR blank is higher in the latter case. The limit of detection, calculated as the copies of plasmid DNA corresponding to a signal which is the PCR blank signal plus three times the standard deviation of the PCR blank signal, results to be 360 copies of plasmid DNA.
Published Version
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