Prevalence of seropositive toxoplasma cases in association with the frequency of abortion in sheep and goat

Abstract

Toxoplasmosis are a serious public-health problem inhuman and animals especially in developing countries, it appear approximately thirty percent from all cases and have higher level of morbidity .At every stage of life the Toxoplasma gondii infections are a significant cause .The epidemiology and handling of this condition are various in the developing world, where infectious agents became predominate .This study was proceed during the period from March 2017 to August 2017 to detect Toxoplasma gondii in sheep and goat serum by characterize them using two assays Latex agglutination test and IgG and IgM Toxoplasmosis ELISA kits.A total of 74 sera samples were collected from sheep and goat from different ages ranged 6 month to 2.5 years old were achieved in different reign in AL- Najaf city / Iraq ,they suffered from endemic abortion and still birth. The overall prevalence of Toxoplasma gondii in were32(62%),18(75%) by Latex agglutination test for sheep and goat, respectively, with non-significant variation (P>0.05) between the two them ,The serum are arranged as follows in a total of 23 (71.8%) and 9 (28.2%)abortion and still birth of sheep ,respectively, and17(94.4%) 1(5.6%) aborted and still birth of goat ,respectively, with significant differences (P≤0.05) between the two them. Out of 32 serum positive of Toxoplasmosis in sheep by using Latex agglutination test, there were 23(71,9%) and 11(43,4%) positive serum by using IgG and IgM ELISA kits, respectively, On the other hand out of 18 toxoplasmosis parasite in goat have positive result in Latex agglutination test, there were 11(61,1%) and 6(33,3%) by using IgG and IgM ELISA kits, respectively, our study was accelerated due to endemic clinical abortion and instill birth for sheep and goat and narrowing functional range of molecular assays by time consumption, in this regard the aim of our study used two functional assay diagnosis Latex agglutination assay and ELISA techniques with increase the accuracy for the detection of the Toxoplasmosis in sheep and goat.