Abstract
Aim::This study was conducted to determine the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in cattle and buffalo and to study their antibiotic resistance pattern.Materials and Methods::A total of 136 samples (skin and nasal swab) from cattle and buffalo were collected. MRSA was identified by conventional bacterial culture techniques which were further confirmed by amplification of S. aureus-specific 16S rRNA by polymerase chain reaction (PCR). The isolates were further analyzed for the presence of mec A gene by PCR. The antimicrobial susceptibility profiling was performed by disc diffusion method.Results::The prevalence of MRSA in the current study was 28.57% and 34.28% in cattle nasal and skin swab, respectively, with an overall prevalence of 31.43% MRSA among cattle. Buffalo nasal and skin sample showed MRSA prevalence of 54.55% and 39.4%, respectively, with 46.9% overall prevalence. PCR could detect mec A gene in 36.4% and 58% MRSA isolates from cattle and buffalo, respectively. Antimicrobial susceptibility test found MRSA resistant to penicillin and oxytetracycline (88% each), cefoxitin (75%), cotrimoxazole (62%), and amoxyclav (50%). 100% sensitivity was observed against ciprofloxacin, amikacin, chloramphenicol, and gentamicin. Three (16.7%) MRSA isolates from buffalo were found resistant to vancomycin.Conclusion::Cattle and buffalo were identified as a potential carrier of MRSA in Bihar (India). The isolation of vancomycin-resistant S. aureus (VRSA) in the current study indicates the emergence of VRSA in animal population which may be transmitted to the human beings working in close contact to the animals.
Highlights
Staphylococcus aureus is an opportunistic pathogen of human and animal [1] which usually colonizes anterior nares and causes infection in immunocompromised patients [2]
polymerase chain reaction (PCR) could detect methicillin resistance (mecA) gene in 36.4% and 58% methicillin resistant S. aureus (MRSA) isolates from cattle and buffalo, respectively
Antimicrobial susceptibility test found MRSA resistant to penicillin and oxytetracycline (88% each), cefoxitin (75%), cotrimoxazole (62%), and amoxyclav (50%). 100% sensitivity was observed against ciprofloxacin, amikacin, chloramphenicol, and gentamicin
Summary
Staphylococcus aureus is an opportunistic pathogen of human and animal [1] which usually colonizes anterior nares and causes infection in immunocompromised patients [2]. Alexander Ogston in 1880 first isolated S. aureus and first isolation of penicillin-resistant S. aureus was made in 1942 from clinical cases [3]. It was later on circumvented by the introduction of methicillin and the first methicillin resistant S. aureus (MRSA) appeared in the year 1961 which later on became a serious nosocomial infection worldwide [4]. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.