Abstract
Pressurized planar electrochromatography (PPEC) of dansyl (DNS) derivatives of amino acids in normal- and reversed-phase systems is presented. The results have been obtained for mobile phases with different acetonitrile (ACN) concentrations (0–85%). The data obtained show differences in separation selectivity between high-performance thin-layer chromatography (HPTLC) and PPEC systems. These differences originate from the electrophoretic effect which is involved in the PPEC system, contrary to the HPTLC one.
Highlights
Amino acids are a significant group of biochemical compounds
High-performance liquid chromatography (HPLC) and capillary electrophoresis techniques are characterized by high efficiency and short separation time [7,8,9,10,11,12]
In previous papers by our group, we reported two-dimensional separation of some amino acids by high-performance thin-layer chromatography (HPTLC) and Pressurized planar electrochromatography (PPEC) on HPTLC RP-18W plates [39] and the comparison of separation selectivity of 20 biogenic amino acids in TLC and PPEC in systems with silica gel and water mobile phase [40]
Summary
Amino acids are a significant group of biochemical compounds. They have many industrial applications, especially in the pharmacy or nutrition industry. Gwarda et al [46,47,48,49,50] presented extensive research on peptides chromatography in HPTLC and PPEC systems with silica and silica-based C18 stationary phases They reported interesting data on the solutes retention and selectivity depending on ion-pairing reagent in the mobile phase [47, 49]. The TFA addition resulted in high electric current and high Joule heat generation during the PPEC process [48] Based on these data, it was interesting to apply analogous systems to separation of DNS amino acids derivatives. JPC – Journal of Planar Chromatography – Modern TLC (2021) 34:105–111 selectivity of DNS amino acids between HPTLC and PPEC systems
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