Abstract
The paper is devoted to the problem of plant biodiversity conservation. This problem is acute all over the world. Lower Don Region has a centuries old history of viticulture and winemaking. There are many valuable vine varieties which are worthy of preservation for future generations. Classical methods no longer cope with this task. Applying advances of biotechnology in addition to traditional methods of ex situ and in situ biodiversity conservation allows sustainable management of genetic resources. This article summarizes the study of methods for creation slow growing collection for grapevine variety Fioletoviy Ranniy. Keeping plants in a slow-growing collection is one of the best ways to preserve biodiversity. This study analyzed the effect of various media compounds on vigor of vine in order to elongate the time between replantings.
Highlights
The problem of biodiversity conservation is not new, but despite the fact that it is paid attention at various levels from international foundations and scientific organizations to small volunteer movements for more than a decade, it is no less relevant.In Russia, this issue is in focus
By Decree of the President of the Russian Federation No 204 of May 7, 2018, the conservation of biological diversity is included in the national goals and strategic objectives of the development of the Russian Federation for the period up to 2024
Our research focuses on the method of preserving of grapevine variety Fioletoviy Ranniy in a slow-growing collection in vitro
Summary
The problem of biodiversity conservation is not new, but despite the fact that it is paid attention at various levels from international foundations and scientific organizations to small volunteer movements for more than a decade, it is no less relevant. Our research focuses on the method of preserving of grapevine variety Fioletoviy Ranniy in a slow-growing collection in vitro. There is an alternative method for storing genetic material – an in vitro collection, which can be used as a backup of field collections for important autochthonous varieties or as independent genetic banks [11,12,13,14] Such collections have many advantages, the main of which are healthy virus-free plants, small occupied areas. The aim of the study is to develop a research protocol for creating an in vitro collection, including introduction to culture in vitro, optimization of the stage of introduction, plant regeneration and selection of parameters that reduce the speed of growth processes to increase the time between replantings. A scheme of introduction to in vitro culture and preparation for deposition was developed, which combines the optimization of introduction, stimulation of regeneration and slowing down the speed of growth processes for the Purple Early variety
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