Abstract
Reacted mixtures obtained by the esterification of three purified fatty acids (oleic acid, icosapentaenoic acid, and docosahexaenoic acid) with glycerol were separated and purified into triacylglycerol, diacylglycerol, monoacylglycerol, and free fatty acid fractions by centrifugal partition chromatography (CPC).Two solvents were used in combination for the separation. One phase was hydrocarbon and the other, a water-miscible solvent. n-Hexane and 85 % ethyl alcohol were used in this study. According to polarity, MG, FFA, and DG were extracted into 85 % ethyl alcohol fractions, and TG was extracted into n-hexane. When mixtures more than 10 g were charged, TG and the other components could be extracted easily with total recovery higher than 90 %.
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